Antifungal properties. The effect of OLE and OPE (10 and
20 g L1) on antifungal properties of CH 20 g L1 edible coating
solution was performed using mycelia yield assay according to
Tripathi, Sharma, and Sharma (2009). PDB medium was prepared
in 50 mL Erlenmeyer
flasks and inoculated with 105 spore mL1 of
P. expansum and R. stolonifer. The
flasks were incubated at 28
1 C
with 120 rpm shaking. After 5 d,
flasks containing mycelia were
filtered through
filter paper Whatman No.1 and washed. The
mycelia were allowed to dry at 60 C for 6 h and at 40 C overnight.
The mycelium dry weight was detected and growth inhibition
percentage was calculated as:
Growthinhibition%
¼
½ðDWutf
DWetf Þ=DWutf
100
ð1Þ
where: DWutf: dry weight of untreated fungal strain and DWetf: dry
weight of treated fungal strain with CH solution and CH-OOR-
incorporated coating solution.