Additionally, an aliquot of the sample was reserved for later protein analysis by AOAC (2012). Thereafter, the
raw milk samples were adulterated using distilled water and the maltodextrin standard to validate this HPLC-ELSD methodology. The addition of 5.0% water was determined based on the minimum amount of water detectable when employing the current official Brazilian analysis routine (density, fat, acidity, total solids and freezing point)