Introduction.Longkong (Aglaia dookkoo Griff.) fruit is a non-climacteric tropical fruit and grows widelyin the South-East Asia. It has a unique taste and nutritional properties that make it more valuable to export. However,longkong exhibits a shorter shelf life at ambient (25◦C for 3−5 days) and low temperature (13◦C for 10 days) storage. Therefore, there is an urgent need to extend its shelf life and marketability by using an inexpensive and proficienttechnique. Material and methods.Methyl jasmonate (MeJA) treatments with different concentrations (10, 20 and30 μMol L−1) were used to control physiological and biochemical quality changes of longkong fruit stored at 13◦C and 85% relative humidity. Fruit with no MeJA treatment served as control. The physiological and biochemical quality analyses were carried out at every four days of the interval. Results and discussion.Longkong pericarp chilling injury (CI) index and ion leakage severity increased in the control fruits as compared with MeJA treated fruit. Theincreased of pericarp phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) activitieswere well controlled by MeJA treatments. Fruit polygalacturonase (PG), pectin methyl esterase (PME) and lipoxygenase (LOX) activities were significantly controlled in MeJA treated fruit. Fruit superoxide dismutase (SOD), catalase(CAT) and glutathione peroxidase (GPX) activities found higher level in the MeJA treated fruit. Conclusion. The different concentrations of MeJA treatment effectively reduced the severity of physiological and biochemical quality changesin longkong fruit under prolonged low temperature storage.