Km and Vmax values of both free and immobilized maltase were calculated using Lineweaver–Burk plot by performing the enzymatic assay using different substrate concentrations. It was found that the Km value of maltase was apparently increased from 1.71 to 3.17 mM ml−1 and Vmax decreased from 8411 U ml−1 min−1 to 4919 U ml−1 min−1 after immobilization