A procedure to quantify intramuscular fat was developed using common inexpensive laboratory equipment.
Three homogenization methods of lyophilized muscle samples (Ball-mill, Grinder and Mortar) and two
extraction methods (Ball-mill or Vortex) were used in turkey meat and pork. Two-hundred mg of lyophilized
and homogenized samples were accurately weighed and mixed with 1.5 mL of dichloromethane–methanol
(8:2) and shaken either in a Mixer Mill (MM400, Retsch Technology) or in a Vortex. The final mixture was
separated by centrifugation. Solvent was evaporated under a nitrogen stream and lipid content was gravimetrically
determined. Besides, it was checked that the fatty acid profile was not altered by the protocol used. Moreover,
the analysis of 4 replicas from the same sample showed different variation coefficients (16–29%) for the
new procedures proposed over a wide range of IMF content. The combination of Grinder and Vortex methodologies
can be proposed as a simple and inexpensive alternative to previous ones.