Four groups of six Holstein calves were inoculated with 3.6 × 107 erythrocytes parasitized with Anaplasma marginale.The criteria for treatment of calves were increasing A. marginale rickettsemia and 30% reduction of baseline packedcell volume (PCV) of each animal. Group 1 (G1) received 7.5 mg.kg–1 of enrofloxacin in a single dose; Group 2 (G2)7.5 mg.kg–1 of enrofloxacin twice every three days; Group 3 (G3) 20 mg.kg–1 of long-acting oxytetracycline in a singledose; and Group 4 (G4) a single dose of PBS. Physical examinations, blood smears and PCV were performed daily.On day treatment, G1, G2 and G3 animals had a mean rickettsemia of 17, 23 and 12%, respectively. At 2 days aftertreatment (DAT) G1 and G2 animals showed a significant reduction of rickettsemia (p < 0.05) compared to G3.G3 animals had high rates of rickettsemia in the first 2 DAT and a slow decrease until stabilization on 9 DAT. The meanPCV in G1 and G2 increased and stabilized after 7 and 8 DAT, respectively. PCV stabilization was achieved in G3 at13 DAT. Both enrofloxacin and oxytetracycline were effective for the treatment of anaplasmosis, but enrofloxacin wasfaster reduction of rickettsemia and PCV recuperation (p < 0.05) compared to oxytetracycline
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