For systemic delivery studies,
4 × 1012 vector genomes were administered in a 200-μl volume of
HBSS via the tail vein. For rapamycin experiments, rapamycin (EMD) was
dissolved overnight in a solution containing 0.2% carboxymethylcellulose sodium salt (Sigma-Aldrich) and 0.25% polysorbate-80 (Sigma-Aldrich)
in water, as described previously (Shioi et al., 2003).