Artemia cysts (GSL strain) were hatched under
the high light intensity in 5 L conical flasks. Before
hatching the cysts were hydrated for three hours in
500 mL of freshwater and 20 ml of bleach was added
in order to disinfect and decapsulate the cysts.
Temperature was maintained at 26°C with vigorous
aeration for 24 hours. Hatched Artemia nauplii were
then cleaned with running freshwater for few minutes
and they were fed yeast until use. After cleaning, the
required numbers of animals were fed to the larvae.