Mass production of Trichoderma harzianum bio-fungicides were done on the substrates rice bran, wheat bran, grass pea bran and their combinations with mustard oilcake (MOC) for the management of seedling rot disease of tomato caused by Rhizoctonia solani in seedbed. Individual and combine use of carrier materials based T. harzianum bio-fungicides were effective in increasing seedling emergence and reducing pre-emergence as well as post-emergence mortality of tomato seedling under R. solani inoculated seedbed condition. Besides, vegetative growth of tomato seedlings viz. shoot length, shoot weight, root length and root weight were enhanced significantly by the different carrier material based T. harzianum bio-fungicides in R. solani sick seedbed condition. The individual (rice bran, wheat bran, grass pea bran) and combination of substrates (rice bran + wheat bran, rice bran + mustard oilcake, rice bran + wheat bran + MOC and wheat bran + grass pea bran + MOC) were equally suitable for mass production of effective T. harzianum bio-fungicides for the management of seedling rot disease of tomato in seedbed condition.