Effect of media
Protocorms about 2-4 mm in length were cultured on
MS and VW supplemented with 15% (v/v) coconut water
(CW) and 2 or 3% (w/v) sucrose. The pH of the medium
was adjusted to 5.5 before adding 8 g/l agar. The culture
vessels were incubated at 25±1°C with a 16 h photoperiod.
The formation of PLBs was determined after 4 weeks
of culture. This part resulted in a medium that was suitable
for the induction of PLBs.