2.5. Antiplasmodial activity calculation and analysis
The antiplasmodial activities of ethanolic extracts were expressed by the inhibitory concentrations (IC50) of the drug that induced 50% reduction in parasitaemia compared to the control (100% parasitaemia).
The IC50 values were calculated (concentration of extract in X axis and percentage of inhibition in Y axis) using Office XP (SDAS) software with linear regression equation[13].
This activity was analyzed in accordance with the norms of antiplasmodial activity of Rasoanaivo et al[14]. According to this norms, an extract is very active if IC50 < 5 毺g/mL, active 5 毺g/mL < IC50 < 50 毺g/mL, weakly active 50 毺g/mL < IC50 100 毺g/mL.
Analysis of variances was performed by ANOVA procedures followed by a specific posthoc test to analyze the difference and statistical significances were achieved when P