• Chemical methods Chemical methods
-Limulus Limulus lysate for endotoxin endotoxin
*Gram – bacteria produce bacteria produce endotoxin endotoxin, which , which
consists of consists of lipopolysaccharide lipopolysaccharide (LPS)
*Limulus Limulus amoebocyte amoebocyte lasate (LAL) obtain (LAL) obtain
from the blood cell of the horseshoe crab from the blood cell of the horseshoe crab
*LAL is sensitive to *LAL is sensitive to endotoxin endotoxin
*adding of food aliquots of food suspension *adding of food aliquots of food suspension
to a lysate portion portion
*incubation, *incubation, endotoxin endotoxin causes the gel causes the gel
formation formation
*E. coli E. coli cells