Specifically, 35 μL of 2,2′-azobis-2-amidinopropane (AAPH, 0.36 mol/L) was added and the fluorescence was measured (ex = 485 nm and em = 535 nm) every minute using a Victor3 multilabel plate reader (Perkin–Elmer, Turku, Finland) maintained at 37 °C until the lecture had declined to less than 5% of the initial reading