The main step of any method to measure the content of RS in
foods must first remove all of the digestible starch from the product
using thermostable -amylases (McCleary and Rossiter 2004). At
present, the method of McCleary and Monaghan (2002) is considered the most reproducible and repeatable measurement of RS in
starch and plant materials, but it has not been shown to analyze all
RS as defined (Champ and others 2003). It is based on the principle
of enzymic digestion and measures the portions of starch resistant
to digestion at 37 °C that are typically not quantitated due to the gelatization
at 100 °C followed by digestion at 60 °C.