Nitric oxide (NO) acts as an important signal molecule with diverse physiological functions in plants. In this study we investigated the effects and possible mechanisms of exogenous NO on anthracnose caused by Colletotrichum gloeosporioides in mango fruit. ‘Guifei’ mango fruit were treated with NO donor (sodium nitroprusside of 0.1 mM) at 25◦C for 5 min, inoculated with spore suspension of C. gloeosporioides after 24 h of NO treatment, and stored at ambient temperature (25◦C). NO treatment effectively suppressed lesion development on mango fruit inoculated with C. gloeosporioides, and lesion diameters at 2 through 8 d in NO-treated fruit averaged 30% lower than those in control fruit.