In order to examine the effect of initial pH on the dye biodegradation, the molasses medium supplied with approximately 25 mg/L dye was prepared at pH 6, 7, 8 and 9. The cells were activated in the media by transferring three times before measuring dye removal. At the predetermined optimum pH value, to acclimatize the microorganisms to high concentrations of dye the cells were gradually exposed to increasing concentrations of dye (from 28.7 to 97.9 mg/L). In addition, control Erlenmeyer flasks containing dye without inoculations were prepared to observe any reaction of media with dye. Batch experiments were performed under aerobic conditions in an orbital shaker (New Brunswick Scientific Innova4230, USA) at an agitation rate of 100 rpm and incubation temperature 30 ± 1◦C. Each experiment was carried out in triplicate. All determinations were made daily during the incubation period. The dye removal properties of the bacteria were investigated in a batch system as a function of initial pH and dye concentrations.