Although the Coulter counter can be considered a flow cytometer, the term flow cytometer is usually reserved for cell counters with fluorescence detectors such as the FACSCanto II(Becton Dickinson) and the Cytomics FC500(Beckman Coulter, Inc. The design of the optical, electronic and computer systems may be somewhat different in various flow cytometers, but they are constructed with essentially the same principles.
With the change of light source from mercury arc to laser, the cooling system from water cooled to air cooled, and the computer system from simple to sophisticated, the size of the flow cytometer has become smaller(table-top) and its function expanded in terms of data storage, number of fluorochromes used, and graphic and data dis However, basic components of a flow cytometer remain the the same: the fluid transport system, the optical system, the electronic system, and the computer system(1-8)
Although the Coulter counter can be considered a flow cytometer, the term flow cytometer is usually reserved for cell counters with fluorescence detectors such as the FACSCanto II(Becton Dickinson) and the Cytomics FC500(Beckman Coulter, Inc. The design of the optical, electronic and computer systems may be somewhat different in various flow cytometers, but they are constructed with essentially the same principles. With the change of light source from mercury arc to laser, the cooling system from water cooled to air cooled, and the computer system from simple to sophisticated, the size of the flow cytometer has become smaller(table-top) and its function expanded in terms of data storage, number of fluorochromes used, and graphic and data dis However, basic components of a flow cytometer remain the the same: the fluid transport system, the optical system, the electronic system, and the computer system(1-8)
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