With large-scale application of cryopreserved sperm, routine hatchery
use and genetic improvement applications are feasible and the value
of sperm is greatly increased. Proper handling and utilization of sperm
also become essential to practice. Because thawed sperm had a larger
variation in motility among individuals than did fresh sperm (Hu
et al., 2011), and testing of the individual fertility of males is typically
not feasible in commercial hatcheries due to time and space limitations,
sperm samples from several blue catfish males are routinely pooled in
commercial hatcheries (Avery et al., 2005). The effects of pooling
spermon subsequent fertilization are not known and could have important
implications for commercial use of cryopreserved sperm. Although
the influence of factors such asmotility and concentration on fertility of
sperm from individual males is becoming more studied (Rurangwa
et al., 2004), it will be important to understand how the pooling of
sperm samples can influence commercial applications. For example,
will aggregate fertilization of the pool be characterized by a few outstanding
individuals, and should sperm of similar quality be pooled
together selectively? The overall goal of this study was to address
high-throughput sperm cryopreservation of blue catfish at a commercial
level. The objectives were to: 1) evaluate double-dosage of thawed
sperm in hybrid fry production; 2) evaluate commercial application of
high-throughput cryopreserved sperm with standard hatchery techniques,
and 3) evaluate the fertility relationship between individuals
and pooled samples.
With large-scale application of cryopreserved sperm, routine hatchery
use and genetic improvement applications are feasible and the value
of sperm is greatly increased. Proper handling and utilization of sperm
also become essential to practice. Because thawed sperm had a larger
variation in motility among individuals than did fresh sperm (Hu
et al., 2011), and testing of the individual fertility of males is typically
not feasible in commercial hatcheries due to time and space limitations,
sperm samples from several blue catfish males are routinely pooled in
commercial hatcheries (Avery et al., 2005). The effects of pooling
spermon subsequent fertilization are not known and could have important
implications for commercial use of cryopreserved sperm. Although
the influence of factors such asmotility and concentration on fertility of
sperm from individual males is becoming more studied (Rurangwa
et al., 2004), it will be important to understand how the pooling of
sperm samples can influence commercial applications. For example,
will aggregate fertilization of the pool be characterized by a few outstanding
individuals, and should sperm of similar quality be pooled
together selectively? The overall goal of this study was to address
high-throughput sperm cryopreservation of blue catfish at a commercial
level. The objectives were to: 1) evaluate double-dosage of thawed
sperm in hybrid fry production; 2) evaluate commercial application of
high-throughput cryopreserved sperm with standard hatchery techniques,
and 3) evaluate the fertility relationship between individuals
and pooled samples.
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