Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A
racemosus is popular for its various medicinal uses such as appetizer, stomachic,
tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot
induction after 3 weeks when Nodal explants segments were cultured in basal
medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal
medium supplemented with BAP + kin were transferred to MS basal medium
containing BAP + Ads and BAP + Kin respectively to the Proliferation shoots.
Stem segments cultured on MS basal medium supplemented with various
concentrations of BAP, AdS and Kin induce shoot, But a combination of BAP +
Kin and BAP + Ads showed the highest percentage of shoot proliferation after 7-14
days. The elongated shoots (3-4 cm length) derived from MS Basal medium
supplemented with BAP & ADS were excised and Transferred to MS medium containing
NAA + BAP + kin and BAP + NAA for rooting. But a combination of NAA + BAP + Kin
showed highest percentage of root formation within7-10 days. Eighty percent of shoots
produced well developed roots in 15-20 days when MS medium was supplemented with
NAA (1.0mg/l)