In plant tissue culture, even though there was a report on safrole production from
embryogenic cultures of nutmeg hitherto, this phytochemical was not a major compound from
the extraction (0.06%) [8]. Besides, aseptic culture of Cinnamomum was still focused mainly on
micropropagation [9], [10], [11], protoplast regeneration [12] and somatic embryogenesis
[13], [14]. As far as we know, the in vitro callus initiation and production of safrole from true
laurel had never been studied yet. Therefore, the aims of this study were to induce callus from
young leaf of true laurel and investigate its ability on safrole production.