Thus, we sayed c-Jun protein expression by immunofluorescence at the wound-healing border of the wound healing scratch assay MDA-MB-231 cells stimulated with either fibroin or sericin. To better quantify cJun expression, fluorescence derived from c pictured as a rainbow quantifying scale. was Upon fibroin or(Figure stimulation, we observed a strong upregulation of c Jun upregulation was even more pronounced as the ells are situated closer to the wound border of the scratch(Figure S4A, B, C). Interestingly, the addition of and PD98059 prevented the fibroin of cJun(Figure 7 B. Similarly, LY294002, SP600125, and blocked serioin induced cJun expression(Figure 7C). These data suggest that cell migration stimulation of fibroin and sericin is related to the expression of oJun at the wound healing edge