The advantages of the method include that it is quite sensitive and is able to detect even 1 µg of protein. Its disadvantages are that it takes rather long to carry out, is disturbed by various materials (including ammonium sulphate, glycine and mercaptans) and that the incubation time is critical. As different proteins contain different amounts of tyrosine, the amount of the coloured product will also be different. As a consequence, this method is more suited to compare the concentration of solutions of the same protein than to absolute measurement.