SSF was carried out in 2 L fermenter containing fermentation medium as described in Section 2.6. Pre-hydrolysis of acid pre- treated wheat straw prior to SSF was performed for 5 h by adding in-house produced cellulase at 10 FPU/g of dry biomass with an agitation of 200 rpm, and temperature and pH were controlled at 50 C and 4.8, respectively.