Triple- and double-layered rotavirus particles can be
separated by centrifugation in sucrose or cesium chloride
(CsCl) gradients. The density of triple-layered particles
is 1.36 g/mL in CsCl and sediment coefficients
are 520S–530S in sucrose. The double-layered particles
have a density of 1.38 g/mL and sediment coefficients
of 380S–400S (Tam et al. 1976). The density and sedimentation
coefficient of single-layered core particles
are 1.44 g/mL in CsCl and 280S, respectively (Bican et
al. 1982).