2.5.2. Reducing power
The reducing power adopted byJung et al. (2010) was used to
measure the antioxidant capacity in the meat. A 200μL aliquot was
mixed with 500μL sodium phosphate buffer (0.2 M, pH 6.6) and
500μL potassium ferricyanide (1%), and the resultant mixture was
incubated at 50 °C for 20 min. After addition of 2.5 mL of 10%v/v
trichloroacetic acid, the mixture was centrifuged (Hanil) at 2200 ×g
for 10 min. The upper layer (500 μL) was mixed with 500μL distilled
water and 100μL ferric-chloride (0.1%). The reaction mixture was
allowed to react for 10 min followed by measuring the absorbance
at 700 nm using a spectrophotometer (Hewlett Packard, UV/visible
light). Increased absorbance of the reaction mixture indicated higher
reducing power of the supplements in the meat samples.