All primers except
MRND5L1 (Clark, 2002) were designed for this study. Sequences
were obtained using an automated sequencer (Applied Biosystems
3130xl). Sequences were aligned and edited using the software
Sequencher v4.8 (Gene Codes). There were no nonsense or stop codons
within the analysed sequences. In cases where the RAG1 gene
was heterozygous (as inferred from double peaks in chromatograms)
at single base positions, the International Union of Pure
and Applied Chemistry (IUPAC) codes for the ambiguous nucleotide
base calls were used. DNA sequences reported in this paper
were deposited in GenBank under accession numbers FJ235624–
FJ235631.