Coleonema album is an aromatic, medicinal and ornamental plant that is in high demand in the traditional medicine
market. In order to meet the increasing demand while conserving those populations in the wild, this study
was aimed at developing an efficient in vitro propagation protocol for C. album. The effect of different cytokinins
[four meta-topolins in comparison with 6-benzyladenine (BA), kinetin (KIN) and thidiazuron (TDZ)] on direct
organogenesis from shoot-tip explants was examined. Each cytokinin was tested at 2.5, 5.0, 7.5 and 10.0 μM.
The effect of combining auxins with the optimal cytokinin concentration on shoot proliferation was also investigated.
At equimolar concentrations, all treatments with BA, meta-topolin (mT, except 10 μM), meta-topolin
riboside and meta-methoxytopolin tetrahydropyran-2-yl significantly increased shoot production when compared
to KIN. The optimum cytokinin concentration for shoot production was 5.0 μM mT with 14.5 shoots regenerated
per explant. The exogenous addition of auxins further increased shoot proliferation (up to 18 shoots per
explant). The findings suggest an additive or synergistic effect of auxin with mT in this study. Regenerated shoots
rooted on half-strength Murashige and Skoog medium supplemented with indole-3-butyric acid (IBA)were successfully
acclimatized with a survival frequency above 70%.
Coleonema album is an aromatic, medicinal and ornamental plant that is in high demand in the traditional medicinemarket. In order to meet the increasing demand while conserving those populations in the wild, this studywas aimed at developing an efficient in vitro propagation protocol for C. album. The effect of different cytokinins[four meta-topolins in comparison with 6-benzyladenine (BA), kinetin (KIN) and thidiazuron (TDZ)] on directorganogenesis from shoot-tip explants was examined. Each cytokinin was tested at 2.5, 5.0, 7.5 and 10.0 μM.The effect of combining auxins with the optimal cytokinin concentration on shoot proliferation was also investigated.At equimolar concentrations, all treatments with BA, meta-topolin (mT, except 10 μM), meta-topolinriboside and meta-methoxytopolin tetrahydropyran-2-yl significantly increased shoot production when comparedto KIN. The optimum cytokinin concentration for shoot production was 5.0 μM mT with 14.5 shoots regeneratedper explant. The exogenous addition of auxins further increased shoot proliferation (up to 18 shoots perexplant). The findings suggest an additive or synergistic effect of auxin with mT in this study. Regenerated shootsrooted on half-strength Murashige and Skoog medium supplemented with indole-3-butyric acid (IBA)were successfullyacclimatized with a survival frequency above 70%.
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