The DPPH assay is based on the measurement of the scavenging ability of an antioxidant using the stable DPPH radical. The free radical DPPH is purple in color in methanol and is reduced to the corresponding hydrazine, which is yellow in color, when it reacts with a hydrogen donor. It is a decoloration assay, which is evaluated by the addition of the antioxidant to a DPPH solution in ethanol or methanol and the decrease in absorbance is measured at 490 nm [22 ,23]. % inhibition=(Absorbance of control– Absorbance of sample) / Absorbance of control ×100