Laccase Laccase activity was determined in reaction
mixture consisting of 1 mM sodium acetate buffer (pH5.0),
0.5 mM 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic
acid (ABTS) and 1.5 mL of culture supernatant. The
absorbance was read at 420 nm and enzyme activity was
defined as amount of enzyme catalyzing production of 1
μmol of colored product per min per mL (Li et al. 2008).