pomegranate concentrate with Brix was with distilled and then bacteria for 5 min at 80 A 24-h cultivation or lactic acid a in the broth was carried out at 30°C. For obtaining of cell density of in the final juice, 15 ml for t the cultivated MRS broth was 4 juice Io mih and the biomass was introduccd into the (150 The juice was then at and sampling was taken every 24 h for microbiological analysis.