In order to study phylogenetic relationship of the pathogen to other Passalora spp and
genetically related genera, ITS sequences were obtained from Genbank, including those of
Passalora spp, Cercospora spp, and Pseudocercospora spp (Table 1). Phylogenetic trees were
constructed using maximum likelihood (ML) and Bayesian analyses of nucleotide sequences.
The general time reversible (GTR) model with six substitution categories was determined to
be the most suitable model with the Modeltest v3.6 software (Posada and Crandall, 1998), and was used for all subsequent nucleotide analyses. ML trees based on nucleotide sequences were
inferred using PHYML v2.4.5 (Guindon and Gascuel, 2003) with an estimated rate distribution shape parameter and bootstrap consensus values calculated using 1000 replicates.
In order to study phylogenetic relationship of the pathogen to other Passalora spp and genetically related genera, ITS sequences were obtained from Genbank, including those of Passalora spp, Cercospora spp, and Pseudocercospora spp (Table 1). Phylogenetic trees were constructed using maximum likelihood (ML) and Bayesian analyses of nucleotide sequences. The general time reversible (GTR) model with six substitution categories was determined to be the most suitable model with the Modeltest v3.6 software (Posada and Crandall, 1998), and was used for all subsequent nucleotide analyses. ML trees based on nucleotide sequences were inferred using PHYML v2.4.5 (Guindon and Gascuel, 2003) with an estimated rate distribution shape parameter and bootstrap consensus values calculated using 1000 replicates.
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