RAW 264.7 cells were pretreated with either HBA, POX nanoparticles
or HPOX nanoparticles for 12 h and then treated with 1 mg
of LPS for 48 h to generate NO. The inhibitory effects of HPOX
nanoparticles on NO production were examined based on the
Griess reaction. Fig. 5 shows the inhibitory effect of HPOX nanoparticles
on the NO production in activated cells. LPS-stimulation
resulted in a large production of NO and the NO concentration
increased up to 18 mM at 48 h post-stimulation. Pretreatment with
62 mg of HBA completely inhibited the LPS-induced NO production.