Temperature dependent Mg2+ permeability.
To elucidate the temperature dependent Mg2+ transport,
Caco-2 monolayers were bathed in 40 mmol/L MgCl2
containing apical solution, while the basolateral solution
had no MgCl2. As shown by the Arrhenius plot (Figure
5E), the ln(PMg) decreased in lower temperatures. The
control monolayers showed Ea of 14.28 ± 1.19 kJ/mol
and Q10 of 1.22 ± 0.04. Fourteen days of 600 ng/mL
omeprazole exposure significantly suppressed Mg2+
transport and increased Ea (19.24 ± 1.98 kj/mol, P <
0.05), but not Q10 (1.31 ± 0.05), of Caco-2 monolayers.