In the new protocol for CMV LAMP, 24 μl reaction mixture containing 1.6 μM each of CMgBFIP and CMgBBIP, 0.2 μM each of outer primer (CMgBF3 and CMgBB3), 0.8 μM each of loop primer (CMgBLPF and CMgBLPB), 2× reaction mix (12.5 μl) and 5 μl of target DNA were incubated at 96◦C for 5 min for heat denaturation.