using
10% running and 4% stacking gel. The molecular mass of proteins was determined using higher range protein molecular weight mar- ker (myosin rabbit muscle 205 kDa, phosphorylase b 97.4 kDa, bovine serum albumin 66 kDa, ovalbumin 43 kDa and carbonic anhydrase 29 kDa) obtained from GeNeiTM, Bangalore, India