Experimental
2.1. Materials
Waxy maize starch (97%) was kindly donated by Ferromet
(Argentina) which commercializes waxy maize starch provided by
Roquette (France). -Amylase from Bacillus licheniformis with a
molecular weight of 62 kDa was purchased from Sigma–Aldrich as
a saline sucrose solution containing 21.0 mg/mL protein (Biuret),
and 623 units/mg protein. d-(+)-Maltose monohydrate standard
and the reagents used in the colorimetric determination of
reducing sugars (potassium sodium tartrate tetrahydrate and 3,5-
dinitrosalicylic acid (DNS)), were all bought from Sigma–Aldrich.
Buffer pH 7 was bought from Laboratorios Olivieri (Argentina). All
other reagents used were of analytical grade.