The starch slurry was passed through a 63 lm sieve and placed at 4 C for 24 h.
The steps of washing with water were repeated three times.
The starch cake was re-suspended in water, neutralized with 1 M hydrochloric acid to pH 7, and stored at 4 C for 24 h.
The supernatant was decanted,and the neutralized starch was re-suspended in water and placed at 4 C for 24 h.
Finally, the supernatant was removed, and the starch cake was dried in an oven at
40 C for 24 h.