Taichung 65 was considered to have lost resistance because the normal BPH26 protein had not been translated due to a nonsense mutation, although gene expression was observed. Gene expressions were also observed in ASD7 and Norin-PL4, both of which have the BPH2 gene. The feeding abilities of BPH on BPH26-NIL and Norin-PL4 were investigated using a BPH2-virulent biotype of BPH. The chi-square test28 revealed that there was no difference between the numbers of survivors on BPH26-NIL and Norin-PL4 (Fig. 4b).