There are 5 main parts in materals and method
Fristly, they collected stool samples from endemic of strogyloidosis areas in Iran.
Second,there are 2 mehods to compare between parasitological methods and molecular methods.
In parasitological mrthods thece are FEC and Agar plate .
And in moleccular method are Nested PCR and Real-time PCR.
In the process of Nested PCR, they have to do DNA isolation.
In the process of Real-time PCR , they have to do sequencing.
Third, In the important process to determine the analytical sentivity of the Nested PCR and Real-time PCR.
Four, the study was reviewed and approved by the Ethics committeesn of Tehran University of Medical Sciance , Iran.
Finally, all data from the experiment would be analyzed by using SPSS