The cultures were incubated under agitation (135 rpm) (mod. Rotabit, Selecta, Spain) at 37 °C until
the stationary growth phase was reached. Before treatment, bacterial suspensions were centrifuged at 6000×g for 5 min at 4 °C and re-suspended in 1 mL of LWE with the corresponding additive at a concentration of approximately 109 CFU/mL. LWE with additives, inoculated with the corresponding microbial population, was immediately subjected to PEF and/or thermal treatments. The additives in the concentrations did not affect the number of survivors during the storage time at 4 °C before the treatments (data not shown).