FPL-Y-049 Compared to P. stipitis strains and other strains of C. shehatae, the FPL-Y-049 C. shehatae strain produced
the most ethanol from the wood hydrolyzate under shake flask conditions. C. shehatae produced more than
20 g/l ethanol from wood hydrolyzate in shake flask
fermentation in 60 h (Fig. 1(a)) whereas P. stipitis produced
15.7 g/l ethanol (Fig. 1(b)). All sugars except arabinose
were assimilated completely (Fig. 1). Traces of
arabitol were produced in the late lag phase of fermentation
(data not shown). Ethanol production by C.
shehatae FPL-Y-049 was not affected by batch-to-batch
feeding of wood hydrolyzate sugars during fermentation
(data not shown).
Ethanol production rates and yields on various
batches of acid hydrolyzates are summarized in Tables
2. Ethanolic fermentation was enhanced in batches of
acid hydrolyzates in which sugars were concentrated
with lower acetic acid levels.