2. Materials and methods
2.1. Experimental materials and rice storage
Three cultivars of milled rice grain (Koshihikari, medium grain
with 18% amylose content; Kyeema, aromatic long grain with
20% amylose content; Doongara, high amylose long grain with
29% amylose content) were used for this study. The cultivars were
grown in the Murrumbidgee Irrigation Area (MIA) of New South
Wales, Australia.
The milled rice was placed in air-tight glass bottles and stored
in the dark at 4 C and 37 C in thermostatically controlled incubators
for 12 months. After storage, samples were withdrawn and the
thermal properties were determined. The rice grains were ground
using a Cyclone Sample Mill (UDY Corporation, Fort Collins, CO)
through a 0.5 mmsieve screen immediately prior to analysis. Moisture
was determined by drying at 110 C to constant weight and
analytical results were expressed on a dry matter basis. All analyses
were performed using triplicate samples.
Protease (Bacillus licheniformis) and cellulase (Trichoderma viride,
EC 3.2.1.4) were purchased from Sigma Chemical Co. (NSW,
Australia). Chemicals and solvent used in this study were either
analytical or high performance liquid chromatography (HPLC)
grade.
2. Materials and methods
2.1. Experimental materials and rice storage
Three cultivars of milled rice grain (Koshihikari, medium grain
with 18% amylose content; Kyeema, aromatic long grain with
20% amylose content; Doongara, high amylose long grain with
29% amylose content) were used for this study. The cultivars were
grown in the Murrumbidgee Irrigation Area (MIA) of New South
Wales, Australia.
The milled rice was placed in air-tight glass bottles and stored
in the dark at 4 C and 37 C in thermostatically controlled incubators
for 12 months. After storage, samples were withdrawn and the
thermal properties were determined. The rice grains were ground
using a Cyclone Sample Mill (UDY Corporation, Fort Collins, CO)
through a 0.5 mmsieve screen immediately prior to analysis. Moisture
was determined by drying at 110 C to constant weight and
analytical results were expressed on a dry matter basis. All analyses
were performed using triplicate samples.
Protease (Bacillus licheniformis) and cellulase (Trichoderma viride,
EC 3.2.1.4) were purchased from Sigma Chemical Co. (NSW,
Australia). Chemicals and solvent used in this study were either
analytical or high performance liquid chromatography (HPLC)
grade.
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