10. Add agar.
11. Heat and stir media on a hot plate (or in microwave after removing the flea/stirrer) till agar is dissolved. Make sure not to overheat/boil the media (heating should be stopped before boiling point).
12. Once agar is fully dissolved, use a media dispenser to distribute 5 ml of media per tubes (Fig. 31). Cover each tube with a plastic cap.
13. Autoclave at 121 oC for 15 minutes- allow slow cooling. See step16.