After 3 days succinate and glutamate were not reduced further
in MAP. The nominal amount of succinate removed during 3 days
was 0.025 mol/kg mince. This value is in agreement with values
suggested by Zhu, Liu, Li, and Dai (2009). The nominal reduction
in glutamate was 0.019 mol/kg mince. It looks like malate is consumed
in MAP, but the metabolism is unaffected (same slope) by
addition of glutamate and succinate. Endogenous glutamate was
low (0.5 mmol/kg) in minces stored in MAP. Endogenous succinate
was substantially higher (5 mmol/kg) than the endogenous
glutamate level. Apparently, the amount of succinate only declined
initially when it needed to remove oxygen, and thereafter this substrate
increased somewhat (Fig. 5A).
After 3 days succinate and glutamate were not reduced furtherin MAP. The nominal amount of succinate removed during 3 dayswas 0.025 mol/kg mince. This value is in agreement with valuessuggested by Zhu, Liu, Li, and Dai (2009). The nominal reductionin glutamate was 0.019 mol/kg mince. It looks like malate is consumedin MAP, but the metabolism is unaffected (same slope) byaddition of glutamate and succinate. Endogenous glutamate waslow (0.5 mmol/kg) in minces stored in MAP. Endogenous succinatewas substantially higher (5 mmol/kg) than the endogenousglutamate level. Apparently, the amount of succinate only declinedinitially when it needed to remove oxygen, and thereafter this substrateincreased somewhat (Fig. 5A).
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