2.5. Pot trial 2
In PT2, soil was spiked with the following silver treatments:
(1) Ag 2 S-NPs; (2) AgNPs; (3) Ag + ; and (4) control (no added
Ag). All Ag treatments were added over 5 days to prevent
waterlogging of the soil. Silver concentrations of the Ag 2 S-NP, AgNP
and Ag + dosed soils were 1.32, 1.20 and 1.32 mg Ag/kg, respectively.
Once the spiked soils were homogenised, 200 g (dry weight) of soil
was added to each pot (n = 4). For each Ag treatment, the agri-
cultural amendments were added, as solutions, to four replicate
pots.
The target soil concentrations of S, P and K after 60 days of
growth were 100 mg (S/P/K)/kg soil for the thiosulfate, phosphate
and KCl fertilisers, respectively (note, the molar concentration of
chloride in soil was equivalent to that of K). The pots receiving
H 2 O 2 treatment were watered to weight with a dilute H 2 O 2 solu-
tion (0.5%) four times over the course of the experiment. See S1.2
for details of fertiliser addition.