Growing media are widely used for strawberry production in protected environments. Due to increasingrestrictions on the use of soil fumigants, there is a need to investigate production of strawberry fruitsin open fields using substrate media. The objectives of the study were to characterize chemical andhydraulic properties of a number of selected growing media mixes and to evaluate them in a two-yearfield study for strawberry production in open fields. The growing media mixes were 100% coconut coir,a peat–perlite (PP) mix, a peat-rice hull (PR) mix, and a peat–coir–rice hull (PCR) mix. Results indicatedthat major macro- and micro nutrient element concentrations, pH, and salinity found in these growingmedia, after supplemental fertilization, were in recommended ranges for strawberry production. Com-pared to field soil, higher water content values were found for these growing media mixes at saturationand after free drainage. Among these media mixes, 100% Coir showed the highest water content valuesat saturation (0.95 cm3cm−3) and after free drainage (0.51 cm3cm−3). The field study also demonstratedthe most vigorous vegetative growth in Coir by showing higher values in plant canopy size and biomassdry weight. In year 1 field experiment, marketable strawberry fruit yields from Coir, PP, and PR averaged41,640 kg ha−1which was not significantly different from the soil beds. Marketable strawberry yieldsfrom the year 2 Coir and PP treatments averaged 48,520 kg ha−1, which was also not significantly dif-ferent from the grower standard practice in field soils. The year 2 yield was higher than year 1 becauseof longer harvesting season and more favorable weather conditions for strawberry growth. The studydemonstrated that certain growing media mixes such as PP or the 100% Coir possess the needed chemi-cal and hydraulic properties for plant growth and the 100% Coir could be used as a promising substratematerial for open-field production of strawberries. Additional work is needed to optimize the irrigationand fertilization regimes to reduce the volume of growing media needed per plant and to evaluate thereuse of the substrate media to reduce production cost.