The rinse fluid (25 mL) was enriched with an equal volume of 2 × blood-free Bolton enrichment broth (Oxoid)
Each sample (50 mL) was incubated at 42 °C for 48 h under microaerobic conditions. A loopful of each of the enriched cultures was streaked onto a mCCDA, mCCtDA, or mCCxDA plate, which were then incubated at 42 °C for 48 h under microaerobic condition.