Based on discordant results of 16 patients, LFA-positive results were mainly obtained from patients who presented with high clinical suspicion for cryptococcal infection and who had already received antifungal treatment along with concordant histopathology, cytology, or fungal culture findings. Patients with false-positive LFA results had lower clinical suspicion for cryptococcal infection. Because of high sensitivity of the LFA, positive cryptococcal antigenemia could be detected over a long period of time. Duration of cryptococcal antigenemia and its association with the disease process have not been well studied. A positive serum LFA result might not reflect acute status or active cryptococcal disease