TG purification and positional analysis. The TG fraction from the
experimental oils was separated by thin layer chromatography as
previously described (40). FAME composition of these TG fractions was
obtained by heating the samples in methanol:toluene:H2SO4 (88:10:2,
by volume) (41). Positional analysis of purified TGs was performed
according to Ruiz-Lopez et al. (26).