2. Materials and methods
2.1. Extraction of active components from common bean seed
The locally obtained common bean (P. vulgaris) dry seed was
ground to a fine powder by using a laboratory mill and sieved
through 0.4 mm sieve. The fraction with particle size less than
0.4 mm was used in experiments. Ten grams of seed powder
was suspended in 1 L of NaCl water solution (0.5 mol/L). The
suspension was stirred using a magnetic stirrer for 10 min to
accomplish extraction and then filtered through a rugged filter
paper (Macherey-Nagel, MN 651/120) to obtain filtrate – crude
extract of active components.